EVALUACIÓN DE LA ACTIVIDAD ANTIINFLAMATORIA Y CITOTÓXICA DE ATRANORINA Y DILACTONA DEL ÁCIDO PULVÍNICO: COMPUESTOS FENÓLICOS BIOACTIVOS DEL LÍQUEN

Diego Vinueza; Marco Janeta; Gisela Pilco; Karen Acosta; Susana Abdo

doi:10.47187/perf.v1i19.24

Authors

Diego Vinueza Laboratory of Natural Products, Faculty of Science, Escuela Superior Politécnica de Chimborazo, Panamericana Sur km 1 1/2, Riobamba, Ecuador
Marco Janeta Department of Pharmacology, Faculty of Pharmacy, University of Valencua, 46100, Burjassot, Valencia-España
Gisela Pilco Laboratory of Natural Products, Faculty of Science, Escuela Superior Politécnica de Chimborazo, Panamericana Sur km 1 1/2, Riobamba, Ecuador
Karen Acosta Laboratory of Natural Products, Faculty of Science, Escuela Superior Politécnica de Chimborazo, Panamericana Sur km 1 1/2, Riobamba, Ecuador
Susana Abdo Laboratory of Natural Products, Faculty of Science, Escuela Superior Politécnica de Chimborazo, Panamericana Sur km 1 1/2, Riobamba, Ecuador

DOI:

https://doi.org/10.47187/perf.v1i19.24

Keywords:

Parmelina tiliacea, lichen, anti-inflamatory activity, cytotoxicity, WST-1

Abstract

Parmelina tiliacea is an Ecuadorian lichen that has been little studied in relation to its potential applications. The research was carried out with the objective of establishing the anti-inflammatory and cytotoxic activity of P. tiliacea, by an in vitro cell method based on the use of a soluble tetrazolium salt (WST-1) on isolated neutrophils. Atranorin and pulvinic dilactone were isolated from lichen P. tiliacea by chromatographic me- thods (flash column chromatography and TLC). The compounds were identified with the help of spectrosco- pic studies (UV and IR), chromatographic (TLC) and specific identification tests for lichen compounds using chemical reagents established for this type of molecules. The results of cytotoxic activity showed a reduction of cell viability up to 15-20% in the presence of a concentration of 200 μg/mL of these compounds. At the same concentration of 200 μg / mL, the anti-inflammatory activity of atranorin, dilactone of pulvinic acid and acetylsalicylic acid (positive control) were respectively 75.92±0.73%, 73.65±0.44% and 83.77±0.47%, respectively. This study represents the basis for subsequent lichen research in Ecuador.

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