Dandelion (Taraxacum officinale) soxhlet extraction process optimization using box-behnken experiment design criteria.

Authors

  • Silvia Toapanta Guanín Universidad Central del Ecuador, Facultad de Ingeniería Química/ Facultad de Ingeniería y Ciencias Aplicadas, Quito, Ecuador
  • Alejandro Delgado Araujo Universidad Central del Ecuador, Facultad de Ingeniería Química/ Facultad de Ingeniería y Ciencias Aplicadas, Quito, Ecuador
  • Jorge Santamaría Carrera Universidad Central del Ecuador, Facultad de Ingeniería Química/ Facultad de Ingeniería y Ciencias Aplicadas, Quito, Ecuador
  • Edison García Narváez Universidad Central del Ecuador, Facultad de Ingeniería Química/ Facultad de Ingeniería y Ciencias Aplicadas, Quito, Ecuador
  • Rita Delgado Araujo Universidad Central del Ecuador, Facultad de Ingeniería Química/ Facultad de Ingeniería y Ciencias Aplicadas, Quito, Ecuador

DOI:

https://doi.org/10.47187/perf.v1i28.190

Keywords:

Box-Behnken Design, Solid-liquid Extraction, Dandelion Leaves, Soxhlet Method, Optimization

Abstract

In Ecuador there is a great variety of medicinal plants that have not been used industrially and one of them is the dandelion plant (Taraxacum officinale). Current research has shown that they have medicinal and food use properties. The study focuses on optimizing the operation parameters in the extraction process by Soxhlet method based on the dried leaves of this plant as raw material. To study the influence of the variables in obtaining the maximum extraction yield, the Box Behnken experimental design is used, where the following factors are considered: extraction time, ethanol concentration, raw material-solvent ratio. Using statistical analysis with the data obtained experimentally, an analysis of variance (ADEVA) was performed, which indicates that the polynomial statistical model is of second order and fits well with a coefficient of determination R2= 74.46. In addition, it was identified that the influence of extraction time is statistically significant in the extraction process. Finally, the optimum conditions in the process were reached as a result of an optimum extraction time of 2h, ethanol concentration 96% (v/v) and a raw material - solvent ratio of 35g/300ml.

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References

Ayala J. Efectividad antimicrobiana del extracto etanólico del Taraxacum officinale (diente de león) al 50% y 100% sobre cepas de Staphylococcus aureus. Universidad Central Del Ecuador; 2017.

Azwanida N. A Review on the Extraction Methods Use in Medicinal Plants, Principle, Strength and Limitation. Med Aromat Plants. 2015;4(3):1–6.

Biel W, Jaroszewska A, Telesiński A. The chemical composition and antioxidant properties of common dandelion leaves compared with sea buckthorn. Can J Plant Sci [Internet]. 2017;97:1–27. Available from: https://doi.org/10.1139/cjps-2016-0409

Calvo L, Tirado D. Extracción de cáñamo. Farm España Ind [Internet]. 2022;40–2. Available from: https://www.farmaindustrial.com/require/archivos/articulos/descarga/B2B0POdg0Op6M2nJEwcEGiono.pdf

Carrillo C, Díaz R, Zambrano J, Águila A, Triana E. Assessment of antioxidant capacity of leaf extracts from three varieties of Mangifera indica L. Rev Cumbres. 2017;3:1–16.

Carrión A, Candidá G. “Preparación de extractos vegetales: determinación de eficiencia de metódica” [Internet]. Universidad de Cuenca; 2010. Available from: https://dspace.ucuenca.edu.ec/bitstream/123456789/2483/1/tq1005.pdf

Fonyuy E, Lambert M, Jeppesen P. Los efectos fisiológicos del diente de león (Taraxacum Officinale) en la diabetes tipo 2. Soc Biomed Diabetes Res. 2016;13.

Gómez M, Jubram J, Reyes M, Kenne A. Inhibitory activity of leaf and bark extracts of the Tree Tetragastris panamensis (Engl.) Kuntze, on in vitro growth of Moniliopthora roreri (cif) H.C.Evans, Stalpers, Samson & Benny. (1978), Biology Laboratory, UNAN- MANAGUA, 2021. Rev Torreón Univ [Internet]. 2022;11:1–11. Available from: http://portal.amelica.org/ameli/journal/387/3873100008/

Guerrero R. Efecto del extracto de diente de león (Taraxacum officinale), sobre el comportamiento productivo y enzimas hepáticas séricas en pollos de engorde [Internet]. Universidad Técnica de Ambato; 2017. Available from: https://repositorio.uta.edu.ec/bitstream/123456789/26208/1/Tesis 89 Medicina Veterinaria y Zootecnia -CD 499.pdf

Gutiérrez H, De la Vara R. Análisis y diseño de experimentos. Segunda Edi. McGRAW-HILL/INTERAMERICANA, editor. México; 2008. 1–564 p.

Hassanat I, Ngoc H. Optimization of ultrasound-assisted extraction conditions for phenolics, antioxidant, and tyrosinase inhibitory activities of Vietnamese brown seaweed (Padina australis). Food Process Preserv. 2021;1–15.

INEN NTE 391. Conservas Vegetales Jugos de Frutas Determinacion de la densidad relativa. 1985;2–5. Available from: https://www.normalizacion.gob.ec/buzon/normas/391.pdf

Jaramillo A, Echavarría A, Hormaza A. Diseño Box-Behnken para la optimización de la adsorción del colorante azul ácido sobre residuos de flores. Ing y Cienc. 2013;9(1794–9165):1–17.

Laquale S, Avato, P, Argentieri, MP, Cándido, V, Perniola, M. Potencial nematicida de Taraxacum officinale. Springer Link. 2018;

Li Q, Yan Z, Zhaolin T. Preparation and antibacterial activity of oligosaccharides derived from dandelion. Rev Int macromoléculas biológicas. 2014;64:1–3.

Montgomery D. Applied Statistics and Probability for Engineers. Seventh Ed. Wiley; 2018. 1–710 p.

Murray M. Taraxacum officinale (Dandelion). In: Natural Medicine. Fifth Edit. 2020. p. 1–4.

Myat T. Study on Botanical Characters and Preliminary Chemical Composition of Dandelion Leaves Taraxacum officinale F.H. Wigg. Myanmar Korea Conf Res J [Internet]. 2019;1–11. Available from: https://www.dagonuniversity.edu.mm/wp-content/uploads/2019/08/26-Myat-Kay-Twe-Lwin-1.pdf

Najihah N, Mohamad R, Musab N. Optimization of Soxhlet Extraction Parameter of Annona Muricata leaves using Box-Behnken Design (BBD) Expert and Antioxidant Analysis. J Teknol (Sciences Eng. 2015;1–12.

Olivera L, Gutierrez E. Evaluación de la actividad antimicrobiana “In vitro” sobre cuatro cepas ATCC y determinación de la actividad antioxidante del extracto etanólico al 96% de Taraxacum officinale (Diente de león) y del aceite esencial de Minthostachys spicata (Q’eshua muña). Universidad Nacional de San Antonio Abad del Cusco; 2021.

Paduret S, Amariei S, Gutt G. The Evaluation of Dandelion (Taraxacum officinale) Properties as a Valuable Food Ingredient. Rom Biotechnol Lett [Internet]. 2016;21. Available from: http://www.rombio.eu/rbl3vol21/16. Sergiu Paduret.pdf

Rasool S, Sharma B. Taraxacum officinale: a high value less known medicinal plant. Ann Plant Sci [Internet]. 2014;(2287-688X):1–8. Available from: %0Awww.annalsofplantsciences.com%0D

Schütz K, Carle R, Schieber A. Taraxacum-a review on its phytochemical and pharmacological profile. Rev Etnofarmacología. 2006;107.

StatPoint I. Diseño de Experimentos – Diseño de Superficie de Respuesta [Internet]. docplayer. 2006. p. 1–15. Available from: https://docplayer.es/20764140-Diseno-de-experimentos-diseno-de-superficie-de-respuesta.html

Suárez M, Rodríguez N. Evaluation of disinfectant action of extracts obtain from Taraxacum officinale y Urtica urens to be used in food industry surfaces. Rev Siembra CBA [Internet]. 2018;1:1–9. Available from: https://revistas.sena.edu.co/index.php/Revsiembracba/article/view/1877

Tello J. Estudio del potencial antiinflamatorio y citotóxico del extracto acuoso de hojas de diente de león (Taraxacum officinale) [Internet]. Universidad Técnica de Ambato; 2018. Available from: https://repositorio.uta.edu.ec/bitstream/123456789/27502/1/BQ 143.pdf

Wang L, Li T, Liu F. Ultrasonic-assisted enzymatic extraction and characterization of polysaccharides from dandelion (Taraxacum officinale) leaves. Rev Int Macromoléculas Biológicas [Internet]. 2018;126:1–49. Available from: https://doi.org/10.1016/j.ijbiomac.2018.12.232

Wu Z, Li Z, Xue Z, Lu X. Optimization and quality evaluation for the extraction technology of phenolic acid from dandelion. Banat J Biotechnol. 2020;XI:1–12.

Yazıcı S. Optimization of all extraction process for phenolic compounds with maximum antioxidant activity from extract of Taraxacum assemanii by statistical strategies. J Food Meas Charact [Internet]. 2021;1–28. Available from: https://doi.org/10.1007/s11694-021-01005-6

Zongxi S, Ruiqiang S, Jianwei Q, Zhiquan Z, Xinsheng W. Flavonoids Extraction from Taraxacum officinale (Dandelion): Optimisation Using Response Surface Methodology and Antioxidant Activity. Hindawi Publ Corp [Internet]. 2014;1–7. Available from: http://dx.doi.org/10.1155/2014/956278%0A

Published

2022-12-31

How to Cite

Toapanta Guanín, S., Delgado Araujo, A., Santamaría Carrera, J., García Narváez, E., & Delgado Araujo, R. (2022). Dandelion (Taraxacum officinale) soxhlet extraction process optimization using box-behnken experiment design criteria. Perfiles, 1(28), 102-110. https://doi.org/10.47187/perf.v1i28.190